Australia's best 23 softballers have entered a Japanese biosecurity bubble, now selectors must determine which eight players will not make the cut for the Olympics.

The softball squad made global headlines last week, becoming the first team to arrive in coronavirus-ravaged Tokyo for the 2021 Olympics.

The Spirit, starved of international fixtures during the past year, had a 3-1 win in their first hit-out on Monday.

Australia will play a stack of tune-ups against local sides, including a chance to test themselves against reigning Olympic champions Japan, before the Games begin on July 21.

Players and staff will remain in Ota City hotel lockdown, only leaving for training and matches, while having daily COVID-19 tests.

It shapes as a particularly onerous five weeks for players left out of a 15-strong squad that will seek to extend Australia's record of winning a medal in every Olympic softball tournament.

"If I don't make the final team it will be hard but I'll want the girls I've been training with to get that gold medal for Australia," Bree Trim told AAP.

"It's a team sport. No matter what happens, you cheer for the other person.

"That's the sort of mentality that all the girls in our team have.

"That (selection dynamic) doesn't mean we become negative towards anyone, we haven't seen that at all in the past year."

Trim and Georgia Hood have been included in the touring party after being overlooked in last year's squad.

The final squad will be almost full of Olympic debutants; captain Stacey Porter and Justine Smethurst are the only players to have previously competed at a Games.

Trim noted her team had a three-week dress rehearsal in Canberra to prepare for bubble life, adding that players feel at ease and have already established a routine that works for them.

"We're shut off from everyone ... I feel just as comfortable here as being at home. Which is great," Trim said.

"We had AIS programs that made us look at different situations and how we'd cope.

"Getting that call-up in January is one of the best feelings I've ever had. Stacey has been one of my biggest inspirations, so to be in a team with her is pretty cool.

"The Japanese people have been so welcoming."

There have been well-documented local complaints about Japan pushing on with the Olympics amid a COVID-19 state of emergency.

But the Spirit have been warmly embraced since touching down at Nartia airport, including Ota mayor Masayoshi Shimizu gushing at a public ceremony they were "so cool ... really cool".